Molecular Biology Fifth Edition

Molecular Biology

Fifth Edition

Robert F. Weaver

University of Kansas

PART I : Introduction

1 A Brief History 1

2 The Molecular Nature of Genes 12

3 An Introduction to Gene Function 30

PART II : Methods in Molecular Biology

4 Molecular Cloning Methods 49

5 Molecular Tools for Studying Genes and Gene

Activity 75

PART III : Transcription in Bacteria

6 The Mechanism of Transcription in Bacteria 121

7 Operons: Fine Control of Bacterial

Transcription 167

8 Major Shifts in Bacterial Transcription 196

9 DNA–Protein Interactions in Bacteria 222

PART IV : Transcription in Eukaryotes

10 Eukaryotic RNA Polymerases and

Their Promoters 244

11 General Transcription Factors

in Eukaryotes 273

12 Transcription Activators in Eukaryotes 314

13 Chromatin Structure and Its Effects

on Transcription 355

PART V : Post-Transcriptional Events

14 RNA Processing I: Splicing 394

15 RNA Processing II: Capping and Polyadenylation 436

16 Other RNA Processing Events and Post-Transcriptional

Control of Gene Expression 471

PART VI : Translation

17 The Mechanism of Translation I: Initiation 522

18 The Mechanism of Translation II: Elongation

and Termination 560

19 Ribosomes and Transfer RNA 601

PART VII : DNA Replication, Recombination,

and Transposition

20 DNA Replication, Damage, and Repair 636

21 DNA Replication II: Detailed Mechanism 677

22 Homologous Recombination 709

23 Transposition 732

PART VIII : Genomes

24 Introduction to Genomics: DNA Sequencing on

a Genomic Scale 759

25 Genomics II: Functional Genomics, Proteomics,

and Bioinformatics 789