Molecular Biology
Fifth Edition
Robert F. Weaver
University of Kansas
PART I : Introduction
1 A Brief History 1
2 The Molecular Nature of Genes 12
3 An Introduction to Gene Function 30
PART II : Methods in Molecular Biology
4 Molecular Cloning Methods 49
5 Molecular Tools for Studying Genes and Gene
Activity 75
PART III : Transcription in Bacteria
6 The Mechanism of Transcription in Bacteria 121
7 Operons: Fine Control of Bacterial
Transcription 167
8 Major Shifts in Bacterial Transcription 196
9 DNA–Protein Interactions in Bacteria 222
PART IV : Transcription in Eukaryotes
10 Eukaryotic RNA Polymerases and
Their Promoters 244
11 General Transcription Factors
in Eukaryotes 273
12 Transcription Activators in Eukaryotes 314
13 Chromatin Structure and Its Effects
on Transcription 355
PART V : Post-Transcriptional Events
14 RNA Processing I: Splicing 394
15 RNA Processing II: Capping and Polyadenylation 436
16 Other RNA Processing Events and Post-Transcriptional
Control of Gene Expression 471
PART VI : Translation
17 The Mechanism of Translation I: Initiation 522
18 The Mechanism of Translation II: Elongation
and Termination 560
19 Ribosomes and Transfer RNA 601
PART VII : DNA Replication, Recombination,
and Transposition
20 DNA Replication, Damage, and Repair 636
21 DNA Replication II: Detailed Mechanism 677
22 Homologous Recombination 709
23 Transposition 732
PART VIII : Genomes
24 Introduction to Genomics: DNA Sequencing on
a Genomic Scale 759
25 Genomics II: Functional Genomics, Proteomics,
and Bioinformatics 789
